Electrochemistry

Biography

Biography: Rajiv Prakash

Abstract

Literature reveals that, in males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types. Prostate specific antigen (PSA) is a 33-kDa serine protease, which is largely bound to endogenous protease inhibitors in human blood serum and its concentration in serum is used as indicator for prostate cancer. In healthy males the PSA concentration level ranges from 0 to 4 ng ml^-1 in the serum. There are several PSA detection methods available like enzyme-linked immunosorbent assay (ELISA), radioimmunoassay, chemiluminescent immunoassay and SPR based immunosensors but are complicated, costly and time consuming. There is urgent need for the development of low cost, user-friendly and quick sensors for PSA. Recently, we have developed a simple and cost-effective biosensor for detection of PSA based on novel graphene quantum dots decorated with gold nanorods (GQDs-AuNRs) and modified with PSA antibody coated over screen-printed electrodes. The detection of PSA is demonstrated using three electrochemical techniques cyclic voltammetry (CV), differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS). A typical response for the PSA is shown in the figure based on EIS technique. The modification of screen printed electrodes with novel hybrid of graphene quantum dots-gold nanorods and simultaneous detection using three different techniques makes the sensor sensitive, reproducible and reliable. PSA immunosensor shows 0.14 ng ml^-1 limit of detection, which is capable of prediction of any disorder or chances of PSA cancer.